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Antibodies from lymphocyte secretions
The antibodies from lymphocyte secretions (ALS) assay is an immunological assay to detect active diseases like tuberculosis, cholera, typhoid etc. Recently, ALS assay nods the scientific community as it is rapidly used for diagnosis of Tuberculosis. The principle is based on the secretion of antibody from ''in vivo'' activated plasma B cells found in blood circulation for a short period of time in response to TB-antigens during active TB infection rather than latent TB infection.
==Procedure of ALS assay==
PBMCs were separated from blood on Ficoll-Paque by differential centrifugation and were suspended in 24-well tissue culture plates culture medium. Different dilutions of PBMCs were incubated at 37°C with 5% CO2. Culture supernatants were collected at 24, 48, 72, and 96 h after incubation and the supernatants were test against BCG or PPD by ELISA. The ELISA titer indicate the positive or negative result.〔Raqib, R., J. Rahman, A. K. Kamaluddin, S. M. Kamal, F. A. Banu, S. Ahmed, Z. Rahim, P. K. Bardhan, J. Andersson, and D. A. Sack. 2003. Rapid diagnosis of active tuberculosis by detecting antibodies from lymphocyte secretions. J. Infect. Dis. 188:364–370〕
* ALS in Tuberculosis Diagnosis is a patent assay of Dr. Rubhana Raqib of International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B).〔http://www.supportforlife.org/media-centre/news/1986-icddrbs-first-patent-a-new-diagnostic-method-for-tuberculo〕〔http://theindependentbd.com/weekly-independent/7817-dr-rubhana-raqib-and-her-new-diagnostic-method-for-tuberculosis.html〕〔http://www.eprothomalo.com/index.php?opt=view&page=1&date=2010-08-01〕
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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